|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5131444||1491308||2017||3 صفحه PDF||سفارش دهید||دانلود کنید|
- MS-CAPS was applied for the rapid detection of RNA virus.
- MS-CAPS could be used in the previously established RT-PCR detection system.
There is a need for rapid and less expensive methods to identify RNA viruses, including luteoviruses, for practical use in agriculture and quarantine. The mass spectrometric cleaved amplified polymorphic sequence (MS-CAPS) method, which detects enzymatically cleaved amplicons by matrix-assisted laser desorption/ionization mass spectrometry, was herein used together with a short RT-PCR to detect luteovirus in only 90Â min. In addition, the matrixes 2â²,4â²,6â²-trihydroxyacetophene and 3-hydroxypicolinic acid were compared for their effectiveness in the analysis of short single-stranded biotinylated DNA obtained by a MS-CAPS reaction.
Journal: Analytical Biochemistry - Volume 539, 15 December 2017, Pages 45-47