کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
5131458 1378750 2017 3 صفحه PDF ندارد دانلود کنید
عنوان انگلیسی مقاله
Recovery and quantification of a myelin oligodendrocyte glycoprotein peptide from rat plasma after protein precipitation
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Recovery and quantification of a myelin oligodendrocyte glycoprotein peptide from rat plasma after protein precipitation
چکیده انگلیسی

The recovery of high molecular weight peptides from complex biological samples is a challenging task. Herein, a reliable, cost effective and rapid methodology was developed for the recovery and quantification of a myelin oligodendrocyte glycoprotein epitope namely (LysGly)5MOG35-55, from rat plasma. Removal of plasma proteins before quantification of the peptide was achieved after precipitation by an acetonitrile/water/formic acid solution. Using the developed protocol, average recoveries of the peptide from plasma ranged between 83.3 and 90.3%.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 538, 1 December 2017, Pages 71-73
نویسندگان
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