|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5131662||1491314||2017||4 صفحه PDF||سفارش دهید||دانلود کنید|
Human norovirus is a leading cause of gastroenteritis worldwide. Although two inÂ vitro cultivation methods have been reported, they cannot provide mechanistic insights into viral inactivation. Receptor-binding assays supplement these assays and give insight into capsid integrity. We present a streamlined version of a receptor-binding assay with minimal time-to-result while maintaining accuracy and high throughput. We validate assay performance for physical and chemical inactivation treatments of a norovirus GII.4 capsid. The assay produces a high positive/negative ratio (25.3Â Â±Â 4.9) in <2.5Â h and has a limit of detection of 0.1Â Î¼g/ml capsid. This method is a valuable additional tool for understanding human norovirus inactivation.
Journal: Analytical Biochemistry - Volume 533, 15 September 2017, Pages 56-59