کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
5131704 1491330 2017 3 صفحه PDF سفارش دهید دانلود کنید
عنوان انگلیسی مقاله ISI
Removal of protein S1 from Escherichia coli ribosomes without the use of affinity chromatography
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موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
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Removal of protein S1 from Escherichia coli ribosomes without the use of affinity chromatography
چکیده انگلیسی


- S1 is removed from ribosomes by centrifugation following incubation with poly(C) or poly(U).
- Size matters: Optimally, the pyrimidine polyribonucleotide is 100-200 nt long.
- The treatment is compatible with standard ribosome washing procedures.
- The expensive affinity resin is no longer needed.

The paper reports an inexpensive and efficient procedure for the removal of protein S1 from E. coli ribosomes. It comprises incubation of ribosomes in a pyrimidine polyribonucleotide solution followed by centrifugation of the sample through a sucrose cushion. To avoid co-sedimentation of the S1-bound polypyrimidine with the ribosomes, its length should not exceed several hundred nucleotides. Unlike popular affinity chromatography through a poly(U) Sepharose or poly(U) cellulose column, the method tolerates limited polyribonucleotide degradation by eventual traces of ribonucleases, and can readily be incorporated into standard protocols for the isolation of ribosomes by centrifugation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 517, 15 January 2017, Pages 53-55
نویسندگان
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