کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5132491 | 1492052 | 2018 | 10 صفحه PDF | دانلود رایگان |
- A novel β-glucan (AAMP-A70) from A. mellea was purified and identified.
- AAMP-A70 increased phagocytosis and secretion of NO, ROS, TNF-α, IL-6 and IL-1β.
- AAMP-A70 activates macrophages through NF-κB and MAPKs pathways via TLR2 receptor.
A novel polysaccharide AAMP-A70 (5.6 kDa) has been purified from the fruiting bodies of Amillariella mellea. Compositional analysis and 1H and 13C NMR spectra indicate that AAMP-A70 is a branched β-glucan with a main chain that consists of β-d-(1â6) linked GlcÏ residues substituted at O-3 by β-GlcÏ or α-d-(1â6)-linked GalÏ side chains. AAMP-A70 increases macrophage phagocytosis and secretion of NO, ROS, TNF-α, IL-6 and IL-1β. Mechanistically, AAMP-A70 promotes degradation of IκB-α and nuclear translocation of the NF-κB p65 subunit, and enhances phosphorylation of MAPKs. In particular, the function blocking antibody to TLR2 substantially suppresses TNF-α and IL-6 production. Our data demonstrate that AAMP-A70 activates macrophages via NF-κB/MAPK signaling pathways and the TLR2 receptor. Overall, AAMP-A70 may serve as a good food supplement to enhance immunity.
Journal: Food Chemistry - Volume 240, 1 February 2018, Pages 534-543