|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5132594||1378816||2018||6 صفحه PDF||سفارش دهید||دانلود کنید|
- Blue mussel protein hydrolysates (BMPH) were produced by enzymatic hydrolysis.
- BMPHÂ <Â 1Â kDa promoted MSCs differentiation into osteoblast.
- Activations of BMP-2 signal pathways by BMPHÂ <Â 1Â kDa were involved in osteoblast differentiation.
- MAPK pathway was also involved in osteoblast differentiation.
Seafood provides a range of health benefits due to its high-protein level. In this study, the osteogenic effect of blue mussel (Mytilus edulis) protein hydrolysates (BMPH) on osteoblast differentiation were examined using mouse mesenchymal stem cells (MSCs). A preparation we called BMPHÂ <Â 1Â kDa which showed the highest osteogenic effect in MSCs, was prepared by peptic hydrolysis. BMPHÂ <Â 1Â kDa treatment stimulated osteoblast differentiation with alkaline phosphatase (ALP) induction, osteocalcin and type I collagen activity as well as calcium deposition. Osteoblast differentiation stimulated by BMPHÂ <Â 1Â kDa treatment was achieved by expression of osteogenic lineage markers, such as bone morphogenetic protein-2 (BMP-2), and downstream signal and transcription factors, including p-Smad1/5/8, Dlx5, runt-related transcription factor 2 (Runx2), and osterix. BMPHÂ <Â 1Â kDa activated phosphorylation of mitogen-activated protein kinases. Adding noggin, a BMP antagonist, inhibited BMPHÂ <Â 1 kDa-induced ALP activity in MSCs. Taken together, our results show that BMPHÂ <Â 1Â kDa promoted osteoblast differentiation by activating BMP-2.
Journal: Food Chemistry - Volume 242, 1 March 2018, Pages 156-161