|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5132687||1378817||2018||6 صفحه PDF||ندارد||دانلود کنید|
â¢Pure and contaminated samples differed in ranges 1423â1123 and 3550â3499Â cmâ1.â¢PCA showed discrete clustering of samples based on level of AFM1.â¢Developed models could correctly classify more than 86% test samples.â¢Developed models could detect as low as 0.02Â Î¼g/l AFM1 in milk (pÂ â©½Â 0.05).â¢ATR-FTIR spectroscopy is a rapid method for assessing AFM1 in milk.
Aflatoxin M1 (AFM1), a potentially carcinogenic compound, is found in milk obtained from animals that consume contaminated feed. Spectra of bovine milk, spiked with AFM1 (0, 0.02, 0.04, 0.06, 0.08 and 0.1Â Î¼g/l) were acquired using attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectrometer. Spectra revealed significant differences among pure and AFM1 spiked samples in spectral regions 1800â650Â cmâ1 and 3689â3499Â cmâ1, which may be attributed to complex chemical structure of AFM1. Principal component analysis (PCA) showed clear clustering of samples (pÂ â©½Â 0.05). The models could successfully classify (>86%) and detect even 0.02Â Î¼g/l AFM1 in milk (pÂ â©½Â 0.05) using SIMCA. AFM1 was best predicted in wavenumber range of 1800â650Â cmâ1 with coefficient of determination (R2) of 0.99 and 0.98, for calibration and validation, respectively, using partial least square (PLS) regression. The study indicated feasibility of ATR-FTIR spectroscopy and chemometrics in rapid detection and quantification of AFM1 in milk.
Journal: Food Chemistry - Volume 238, 1 January 2018, Pages 209-214