کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
5132687 1378817 2018 6 صفحه PDF ندارد دانلود کنید
عنوان انگلیسی مقاله
Detection of aflatoxin M1 in milk using spectroscopy and multivariate analyses
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Detection of aflatoxin M1 in milk using spectroscopy and multivariate analyses
چکیده انگلیسی

•Pure and contaminated samples differed in ranges 1423–1123 and 3550–3499 cm−1.•PCA showed discrete clustering of samples based on level of AFM1.•Developed models could correctly classify more than 86% test samples.•Developed models could detect as low as 0.02 μg/l AFM1 in milk (p ⩽ 0.05).•ATR-FTIR spectroscopy is a rapid method for assessing AFM1 in milk.

Aflatoxin M1 (AFM1), a potentially carcinogenic compound, is found in milk obtained from animals that consume contaminated feed. Spectra of bovine milk, spiked with AFM1 (0, 0.02, 0.04, 0.06, 0.08 and 0.1 μg/l) were acquired using attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectrometer. Spectra revealed significant differences among pure and AFM1 spiked samples in spectral regions 1800–650 cm−1 and 3689–3499 cm−1, which may be attributed to complex chemical structure of AFM1. Principal component analysis (PCA) showed clear clustering of samples (p ⩽ 0.05). The models could successfully classify (>86%) and detect even 0.02 μg/l AFM1 in milk (p ⩽ 0.05) using SIMCA. AFM1 was best predicted in wavenumber range of 1800–650 cm−1 with coefficient of determination (R2) of 0.99 and 0.98, for calibration and validation, respectively, using partial least square (PLS) regression. The study indicated feasibility of ATR-FTIR spectroscopy and chemometrics in rapid detection and quantification of AFM1 in milk.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 238, 1 January 2018, Pages 209-214
نویسندگان
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