کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5133390 | 1492060 | 2017 | 9 صفحه PDF | دانلود رایگان |
- XynM is a fungal GH11 xylanase pH- and Cu2+-tolerant from Talaromyces amestolkiae.
- Enzymatic hydrolysis of birchwood xylan yielded 28.8% neutral XOS.
- The whole mix of XOS had prebiotic effects on breast-fed infant feces fermentations.
- Bifidogenic and anti-pathogenic activities were noticed.
- Growth induction of the potential probiotic Staphylococcus hominis was outstanding.
A fungal endoxylanase belonging to the glycoside hydrolase gene family 11 (GH11) was obtained from the ascomycete Talaromyces amestolkiae. The enzyme was purified, characterized and used to produce a mixture of xylooligosaccharides (XOS) from birchwood xylan. A notable yield of neutral XOS was obtained (28.8%) upon enzyme treatment and the mixture contained a negligible amount of xylose, having xylobiose, xylotriose and xylotetraose as its main components. The prebiotic potential of this mixture was demonstrated upon analyzing the variations in microorganisms' composition and organic acids profile in breast-fed child faeces fermentations. The strong production of acetic and lactic acid, the decrease of potentially pathogenic bacteria and the increase of bifidobacteria, and possible beneficial commensals, confirmed the prebiotic value of these xylooligosaccharides.
Journal: Food Chemistry - Volume 232, 1 October 2017, Pages 105-113