|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5133973||1378836||2017||7 صفحه PDF||ندارد||دانلود کنید|
â¢Hydrolysis of oleuropein with different enzymes.â¢Selective ultrasound-assisted enzymatic hydrolysis of oleuropein to its aglycon.â¢Drastic shortening of time for hydrolysis vs conventional enzymatic incubation.
Hydrolysis of oleuropein, the main phenol in olive (Olea europaea L.) leaf extracts, to oleuropein aglycon and other subsequent products in the hydrolytic pathway can be catalyzed by different enzymes. Three of the most used hydrolases were assayed to catalyze the process, and Î²-glucosidase from Aspergillus niger was selected. Acceleration of the enzymatic hydrolysis by ultrasound (US) was studied using a Box-Behnken design (duty cycle, amplitude, cycle time) and an oleuropein standard, and the optimum US conditions for achieving maximum yield of oleuropein aglycon were 0.5Â s/s duty cycle, 50% amplitude and 45Â s cycle. The method was applied to obtain oleuropein aglycon from commercial and laboratory extracts from olive leaves, which may have a pharmacological use as deduced by its healthy properties. The kinetics of the US-assisted enzymatic hydrolysis was monitored by analysis of the target compounds using liquid chromatographyâtandem mass spectrometry.
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Journal: Food Chemistry - Volume 220, 1 April 2017, Pages 282-288