Rapid quantitative detection of glucose content in glucose injection by reaction headspace gas chromatography

- Rapid quantifying the glucose content in glucose injection.
- This method is based on the oxidation reaction between glucose in glucose injection and potassium dichromate under the acidic conditions.
- The carbon dioxide (CO2) formed from the oxidation reaction can be quantitatively detected by gas chromatography.
- The HS-GC method is rapid, practical and can be utilized for quantitatively analyzing the glucose content in glucose injection related applications.

This work investigates an automated technique for rapid detecting the glucose content in glucose injection by reaction headspace gas chromatography (HS-GC). This method is based on the oxidation reaction of glucose in glucose injection with potassium dichromate. The carbon dioxide (CO2) formed from the oxidation reaction can be quantitatively detected by GC. The results show that the relative standard deviation (RSD) of the present method was within 2.91%, and the measured glucose contents in glucose injection closely match those quantified by the reference method (relative differences <6.45%). The new HS-GC technique is rapid, practical and can be used to the batch detection of the glucose content in glucose injection related applications.