کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5136074 | 1493997 | 2017 | 6 صفحه PDF | دانلود رایگان |
- Developed a method to determine brigatinib by UPLC-MS/MS.
- This method was reliable, specified, stable and rapid.
- This method can be used in pharmacokinetic studies.
Brigatinib is the second-generation anaplastic lymphoma kinase - inhibitor in non-small cell lung cancer and it can overcome the crizotinib-resistance. Chromatographic separation was carried out on an Acquity Ultra Performance Liquid Chromatography (UPLC) unit with a BEH C18 column (2.1 mm Ã 50 mm, 1.7 μm). The mobile phase was composed of acetonitrile and 0.1% formic acid in water. No endogenous interfering compounds was discovered at retention time of brigatinib (0.56 min) and imatinib (IS, 1.41 min). MS/MS detection was performed in positive mode. And the MRM transitions were m/z 584.09 â 484.08 and m/z 494.3 â 394.2 for brigatinib and IS, respectively. This method was assessed to be stable, specificity, and no matrix effect in three concentrations (0.004, 0.4, 4 μg/mL). The intra-day and inter-day precisions were less than 11.09% and 6.43%. And intra-day and inter-day accuracies were ranged from â3.88% to 5.44%. The recovery of brigatinib was from 85.26% to 96.05%. Additionally, the method had a good linearity in the range of 0.002-5 μg/mL. The presented method was effectively implemented to determine the concentration of brigatinib in rat plasma.
Journal: Journal of Chromatography B - Volumes 1068â1069, 15 November 2017, Pages 84-89