کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5136530 | 1494016 | 2017 | 7 صفحه PDF | دانلود رایگان |
- Crocin and crocetin were sensitively determined in rat plasma using LC/MS.
- Pharmacokinetic properties of crocin and crocetin was evaluated.
- Crocetin in plasma was much higher than crocin after orally given with crocin.
- Oral administration of crocin has the advantages over crocetin.
- Crocetin is the active component contributing to pharmacological effect of crocin.
Crocin and crocetin in rat plasma were simultaneously analysed using ultra-performance liquid chromatography tandem mass spectroscopy (UPLC-MS/MS), and method was fully validated. For the first time, levels of both crocin and crocetin in plasma were profiled after oral administration of crocin, and this UPLC-MS/MS approach was applied to evaluate pharmacokinetics and relative bioavailability of crocin and crocetin in rats. It was shown that crocin transformed into crocetin quickly in the gastrointestinal tract, and crocetin was 56-81 fold higher exposed in rat plasma than crocin after oral administration of crocin. A comparison study revealed that an oral administration of equal molar crocin achieved higher exposure of crocetin in rat plasma than that of crocetin. It was suggested that oral administration of crocin has the advantages over crocetin, and crocetin may be the active component potentially responsible for the pharmacological effect of crocin.
Journal: Journal of Chromatography B - Volumes 1044â1045, 15 February 2017, Pages 1-7