کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5136665 | 1494021 | 2016 | 6 صفحه PDF | دانلود رایگان |
- A novel method for quantifying fisetin and geraldol using LC-MS/MS was developed and validated.
- The method was successfully used in a pharmacokinetic study of fisetin and geraldol in mice.
- Geraldol was the dominant circulating metabolite after fisetin administration in vivo.
Fisetin (3,3â²,4â²,7-tetrahydroxyflavone) is a flavonoid found in several fruits, vegetables, nuts, and wine and has anti-oxidant, anti-inflammatory, and anti-angiogenic properties. Geraldol is the 3â²-methoxylated metabolite of fisetin (3,4â²,7-trihydroxy-3â²-methoxyflavone). The concentration of fisetin and geraldol in mouse plasma was determined by LC-MS/MS, following direct protein precipitation. These concentrations were determined after administration of fisetin at doses of 2Â mg/kg (i.v.) and 100 and 200Â mg/kg (p.o.). The method was validated in terms of linearity, accuracy, precision, matrix effect, and stability. The pharmacokinetics parameters of fisetin and geraldol were successfully determined using a validated method in mice. Results indicated that fisetin was very rapidly methylated to geraldol in vivo. Following administration of fisetin, it was observed that the Cmax and AUC values for geraldol were higher than those of fisetin. The absolute bioavailability of fisetin was calculated as 7.8% and 31.7% after oral administration of 100 and 200Â mg/kg fisetin, respectively. This method was successfully applied to determine the pharmacokinetic parameters of fisetin and its main metabolite geraldol in mouse plasma. Geraldol was the dominant circulating metabolite after fisetin administration in vivo.
Journal: Journal of Chromatography B - Volume 1038, 1 December 2016, Pages 95-100