کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5137125 | 1494533 | 2017 | 9 صفحه PDF | دانلود رایگان |
- EPS-producer Leu. citreum L3C1E7 strain was tested for immunomodulatory action.
- Leu. citreum L3C1E7 reduced IL-8 release by LPS-induced HT-29 cells.
- Orally administered live strain modulate the systemic immune response in rats.
- The strain down-regulated the over-production of IgE, in a rat-induced asthma model.
The present study aimed to assess the immunomodulatory properties of Leuconostoc citreum L3C1E7, isolated from Pico cheese. In vitro tests showed a significant (PÂ <Â 0.05) effect of live strain on reducing pro-inflammatory cytokine IL-8 secretion by lipopolysaccharide-induced HT-29 cells. The probiotic activity of the strain was further investigated in two rat models. Rats fed with ovalbumin (OVA), with/without the strain, were vaccinated subcutaneously with OVA on day 7 and 14. Allergen-specific IgG1 and IgG2a responses were restored after pre-feeding the strain. In a second experiment, an OVA-induced asthma model was used. Plasma levels of allergen-specific IgG1 and IgG2a were significantly (PÂ <Â 0.05) raised in the OVA-fed group, while co-feeding with the strain significantly (PÂ <Â 0.05) reduced plasma levels of these antibodies. Moreover, plasma OVA-specific IgE was significantly (PÂ <Â 0.05) reduced by feeding the strain. These data show that feeding Leu. citreum L3C1E7 suppresses allergen-specific IgE synthesis and may alleviate Th2-mediated allergic symptoms.
Journal: Journal of Functional Foods - Volume 33, June 2017, Pages 235-243