کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5137177 | 1494534 | 2017 | 12 صفحه PDF | دانلود رایگان |
- LC-AX modulated lumen and mucus microbiota of proximal colon (M-SHIME).
- LC-AX modulated gut microbiota, consistent with inter-individual variations.
- LC-AX increased Bifidobacterium in both lumen and mucus compartments from M-SHIME.
- LC-AX had a potential prebiotic effect in the mucus layer.
- High dose of LC- AX increased propionate in the in vitro proximal colon.
Prebiotic compounds aim at improving human health by targeting beneficial gut microbes. We used a dynamic in vitro model of the human digestive tract (M-SHIME®) to study the modulatory effects of long-chain arabinoxylans (LC-AX) towards luminal and mucosal microbiota. Two doses (3 and 6 g/L) of LC-AX were supplied to the proximal colon compartments of the M-SHIME, three times per day for 3 days. Next generation sequencing and multivariate statistical analysis revealed the distribution of key microbial genera across M-SHIME compartments. Supplementation of 6 g/L of LC-AX significantly increased the relative abundance of Bifidobacterium in both lumen (from 0.34% to 0.41%, 1.2-fold) and mucus (from 0.14% to 0.29%, 2.1-fold) compared with the control (P < 0.001). Propionate (1.4-fold), as well as activity of β-xylanase, β-xylosidase and α-arabinofuranosidase (P < 0.05) were also increased in the lumen. LC-AX may potentially benefit host health by stimulating Bifidobacterium abundance and its metabolic activity.
Journal: Journal of Functional Foods - Volume 32, May 2017, Pages 226-237