کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5137278 1494531 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of apple extracts and selective polyphenols on the adhesion of potential probiotic strains of Lactobacillus gasseri R and Lactobacillus casei FMP
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Effect of apple extracts and selective polyphenols on the adhesion of potential probiotic strains of Lactobacillus gasseri R and Lactobacillus casei FMP
چکیده انگلیسی


- Apple peel contained significantly higher amounts of polyphenols than apple pulp.
- Apple peel, but not pulp, extracts improved lactobacillus epithelial cell adhesion.
- Phloretin showed highest toxicity towards Caco-2 and HT29-MTX cells.
- Quercetin and its glycosides were the most potent in promoting bacterial adhesion.

We aimed to investigate the influence of apple extracts (pulp and peel) and their phenolic constituents on the adhesion of two lactobacillus strains to Caco-2 and HT29-MTX intestinal epithelial cells, which were used as models of gastrointestinal tract mucosa. Apple pulp extracts (10 and 20 µg/mL) decreased Lactobacillus gasseri R adhesion by 39.7% (P < 0.05) and 45.9% (P < 0.05), and Lactobacillus casei FMP adhesion by 6.8% and 19.4%, respectively. In contrast, apple peel (10 µg/mL) enhanced L. gasseri R adhesion by 35.7% (P < 0.05) and L. casei FMP adhesion by 28.2% (P < 0.05). Apple peel at 20 µg/mL increased the adhesion of both strains.Phenolic profiles of the extracts were characterised using high-resolution and accurate mass quantitative analysis; procyanidin B2 and chlorogenic acid were the main constituents. When tested individually, all the phenolics increased the adhesion of both lactobacilli. Our data indicated that apple peel and its phenolic constituents affected the microbiota-epithelium interaction.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Functional Foods - Volume 35, August 2017, Pages 391-397
نویسندگان
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