کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5138370 | 1494601 | 2017 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Liquid chromatography-tandem mass spectrometric determination of propofol in rat serum and hair at attogram level after derivatization with 3-bromomethyl-propyphenazone
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
EugEugenol - اوژنولSerum - سرمTriple quadrupole mass spectrometer - سهم طیف سنج جرمی چهارگانهIon trap mass spectrometer - طیف سنج جرم تله یونBMP - مدیریت فرایند کسب و کارDerivatization - مشتقاتHair - موPRO - نرم افزارPropofol - پروپوفولliquid chromatography tandem mass spectrometry - کروماتوگرافی مایع اسپکترومتری دو طرفه
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A sensitive, selective and precise liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for determination of propofol (PRO) in rat serum and hair has been developed. 3-Bromomethyl-propyphenazone was used as derivatization reagent forming propofol-methyl-propyphenazone compound. The derivatization reaction was optimized and validated for maximum MS sensitivity. The MS instrumental sensitivity reached to 10 attogram. The serum samples were extracted by using Chromabond C8 columns, while hair samples extracted with methanol. The tendency of volatility of PRO was minimized by adding triethylamine to the extract before the use of nitrogen gas for evaporation of solvent. The limit of quantitation (LLOQ) was 0.01Â pg/mL and the assay was linear to 10000Â pg/mL. The intra-and inter-day precision (RSD%) ranged from 0.33 to 3.44% while the accuracy (Er%, relative error) were â6.4 to 1.1%. The ionization suppression, due to reagent, was minimized by reacting the excess reagent with methanol, and eluting to waste before MS ionization source (2-4.5Â min). The method was successfully applied for detection and determination of PRO in rat serum and hair after 7-28Â days from administration of only one dose of propofol (10Â mg/kg).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 134, 5 February 2017, Pages 195-202
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 134, 5 February 2017, Pages 195-202
نویسندگان
Alaa Khedr, Soad S.Abd El-Hay, Ahmed K. Kammoun,