کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5140557 1495762 2017 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An interference-free and label-free sandwich-type magnetic silicon microsphere -rGO-based probe for fluorescence detection of microRNA
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
An interference-free and label-free sandwich-type magnetic silicon microsphere -rGO-based probe for fluorescence detection of microRNA
چکیده انگلیسی
An interference-free and label-free sensing platform was developed for microRNA-21 detection. DNA capture probes (P1) were connected with MNPs and hybridized with completely complementary target miRNA. Subsequently, rGO was retained and induced the fluorescence quenching in the supernatant. When DNA capture probe-modified magnetic silicon microspheres (MNP-P1) were adsorbed through rGO in the absence of a target and formed a sandwich structure, the formed nanostructure was easily removed from the solution by a magnetic field and the fluorescence intensity was maximally recovered.86
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 174, 1 November 2017, Pages 679-683
نویسندگان
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