کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5144955 | 1496894 | 2018 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
High frequency electroporation efficiency is under control of membrane capacitive charging and voltage potential relaxation
ترجمه فارسی عنوان
راندمان الکتریکی با فرکانس بالا تحت کنترل شارژ خازنی غشا و آرام سازی توان بالقوه است
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
الکتروشیمی
چکیده انگلیسی
The study presents the proof of concept for a possibility to achieve a better electroporation in the MHz pulse repetition frequency (PRF) region compared to the conventional low frequency protocols. The 200 ns Ã 10 pulses bursts of 10-14 kV/cm have been used to permeabilize Chinese hamster ovary (CHO) cells in a wide range (1 Hz-1 MHz) of PRF. The permeabilization efficiency was evaluated using fluorescent dye assay (propidium iodide) and flow cytometry. It was determined that a threshold PRF exists when the relaxation of the cell transmembrane potential is longer than the delay between the consequent pulses, which results in accumulation of the charge on the membrane. For the CHO cells and 0.1 S/m electroporation medium, this phenomenon is detectable in the 0.5-1 MHz range. It was shown that the PRF is an important parameter that could be used for flexible control of electroporation efficiency in the high frequency range.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioelectrochemistry - Volume 119, February 2018, Pages 92-97
Journal: Bioelectrochemistry - Volume 119, February 2018, Pages 92-97
نویسندگان
Vitalij Novickij, Paulius Ruzgys, Audrius Grainys, Saulius Å atkauskas,