کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5156789 | 1500584 | 2017 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Optimization of dextran production by Weissella cibaria NITCSK4 using Response Surface Methodology-Genetic Algorithm based technology
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The most influencing factor on dextran production by Weissella cibaria NITCSK4 were screened using Plackett Burman design at 95% confidence limit with higher value of co-efficient of determination (R2) 99.58%. The combined effects of significant factors, namely, sucrose, temperature, dipotassium hydrogen phosphate (K2HPO4) and yeast extract were studied and optimized using Response Surface Methodology (RSM). The input parameters of non-linear models predicted by RSM were subsequently optimized using the genetic algorithm (GA) for obtaining a maximum dextran yield. The maximum yield was obtained with sucrose concentration of 15.78%, yeast extract 1.27%, K2HPO4 1.25%, and at 26 °C. The predicted conditions were experimentally validated and 43.79 mg/ml of dextran was produced. The dextran yield was 51% higher as compared to unoptimized medium. The molecular weight of resulting dextran produced at 26 °C is >2000 kDa. The NMR spectroscopic analysis demonstrated that the NITCSK4 produced linear dextran with predominant α (1-6) linkage.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 174, 15 October 2017, Pages 103-110
Journal: Carbohydrate Polymers - Volume 174, 15 October 2017, Pages 103-110
نویسندگان
J. Kanimozhi, I. Ganesh Moorthy, R. Sivashankar, V. Sivasubramanian,