کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
5324 366 2016 15 صفحه PDF ندارد دانلود رایگان
عنوان انگلیسی مقاله
A high affinity kidney targeting by chitobionic acid-conjugated polysorbitol gene transporter alleviates unilateral ureteral obstruction in rats
ترجمه فارسی عنوان
هدف گیری کلیه با میل ترکیبی بالا توسط انتقال دهنده ژن polysorbitol اسید کونژوگه chitobionic سبب برطرف شدن انسداد حالب در موش صحرایی می شود
کلمات کلیدی
اسید Chitobionic؛ انتقال دهنده ژن Polysorbitol؛ HGF؛ هدف گیری کلیه . UUO
Chitobionic acid; Polysorbitol gene transporter; HGF; Kidney targeting; UUO
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی

Aside from kidney transplantation – a procedure which is exceedingly dependent on donor-match and availability leading to excessive costs – there are currently no permanent treatments available which reverse kidney injury and failure. However, kidney-specific targeted gene therapy has outstanding potential to treat kidney-related dysfunction. Herein we report a novel kidney-specific targeted gene delivery system developed through the conjugation of chitobionic acid (CBA) to a polysorbitol gene transporter (PSGT) synthesized from sorbitol diacrylate and low molecular weight polyethylenimine (PEI) carrying hepatocyte growth factor (HGF) gene to alleviate unilateral ureteral obstruction (UUO) in rats. CBA-PSGT performed exceptionally well for targeted delivery of HGF to kidney tissues compared to its non-targeted counterparts (P < 0.001) after systemic tail-vein injection and significantly reduced the UUO symptoms, returning the UUO rats to a normal health status. The kidney-targeted CBA-PSGT-delivered HGF also strikingly reduced various pathologic and molecular markers in vivo such as the level of collagens (type I and II), blood urea nitrogen (BUN), creatinine, and the expressions of ICAM-1, TIMP-1 and α-SMA which play a critical role in obstructive kidney functions. Therefore, CBA-PSGT should be further investigated because of its potential to alleviate UUO and kidney-related diseases using high affinity kidney targeting.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 102, September 2016, Pages 43–57
نویسندگان
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