کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5370695 1503901 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Relocation of a biological photosensitizer from non-ionic micellar carrier to DNA: A multispectroscopic investigation
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
Relocation of a biological photosensitizer from non-ionic micellar carrier to DNA: A multispectroscopic investigation
چکیده انگلیسی


- Binding interactions of PSF with TX-100 micelle and ctDNA have been investigated independently.
- In the presence of DNA, transfer of PSF from the micellar environment to the DNA occurs.
- Competitive binding is ascribed to be responsible for the relocation of the probe.
- TX-100 micelle does not affect the structure of the ctDNA.
- TX-100 micelle can be used as a safe carrier for the delivery of drugs to DNA.

Relocation of a bioactive photosensitizer, namely phenosafranin (PSF), from the phenazinium family, has been demonstrated from non-ionic micellar carrier to the DNA. For the purpose, interaction of micelle-bound PSF with calf thymus DNA (ctDNA) has been investigated vividly exploiting various spectroscopic techniques like absorption, steady state and time resolved emission, fluorescence anisotropy, circular dichroism etc. Experimental outcomes reveal that PSF binds strongly with both the micelle as well as the DNA. In the presence of DNA, however, relocation of the micelle-carried PSF occurs from the micelle to the DNA. Competitive binding of the probe between micelle and the DNA is assigned responsible for this relocation. Circular dichroism spectral measurements reflect that the DNA conformation remains intact in the presence of the micelle advocating that the non-ionic micelles can safely be used for the drug delivery purpose. The work is expected to encourage development of newer carriers for DNA targeted drug delivery.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biophysical Chemistry - Volume 219, December 2016, Pages 75-81
نویسندگان
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