کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5370771 | 1503908 | 2016 | 11 صفحه PDF | دانلود رایگان |
- The stabilization effect of sucrose on denaturation of domain-I of HSA is studied.
- The water dynamics found to play a crucial role in the stabilization process at low GnHCl concentration.
- Environmental restriction turned out to be the key stabilization factor for higher concentrations of GnHCl.
The present study is devoted to understand the effect of sucrose on the hydration dynamics and rotational relaxation dynamics within the domain-I of HSA during chemically as well as thermally induced unfolding. It has been observed that the average solvation time become slower in the presence of sucrose for the lower concentrations of GnHCl, however at higher concentrations of GnHCl the effect of sucrose is almost negligible. From the time resolved fluorescence anisotropy it has been observed that in the lower concentration region of GnHCl the sucrose induced stabilization is small as compared to the higher concentrations of GnHCl. We have concluded that the hydration dynamics plays an important role in the sucrose induced stabilization process at the low concentration region; whereas environmental restriction is responsible at the higher concentration of GnHCl. However, we have observed a negligible stabilizing effect of sucrose towards the temperature induced unfolding.
327
Journal: Biophysical Chemistry - Volume 211, April 2016, Pages 59-69