Photo-dynamics of the BLUF domain containing soluble adenylate cyclase (nPAC) from the amoeboflagellate Naegleria gruberi NEG-M strain

► Photo-activated adenylyl cyclase (nPAC) from Naegleria gruberi NEG-M was expressed. ► Photodynamics of BLUF domain in BLUF sensor - cyclase actuator protein was studied. ► Photo-excitation caused BLUF photo-cycling and permanent protein re-conformation. ► Re-conformed protein enabled photo-induced flavin reduction by proton transfer. ► Fluorescence of flavin in dark- and light-adapted state of nPAC was characterized.