کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5395559 1505715 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Surface characterization of insulin-coated Ti6Al4V medical implants conditioned in cell culture medium: An XPS study
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
Surface characterization of insulin-coated Ti6Al4V medical implants conditioned in cell culture medium: An XPS study
چکیده انگلیسی
Surface characterization of insulin-coated Ti6Al4V medical implants, after incubation in α-minimum essential medium (α-MEM), was done by X-ray photoelectron spectroscopy (XPS), in order to analyze the insulin behavior at the implant - α-MEM interface. In the absence of serum proteins in cell culture medium, the coated insulin layer remained intact, but experienced a time-dependent structural transformation exposing hydrophobic parts of the protein toward the solution. The presence of fetal bovine serum (FBS) in the medium resulted in partial substitution of insulin by serum proteins. In spite of some insulin release, the remaining coated layer demonstrated a direct surface effect by stabilizing the structure of protein competitors, and by supporting the accumulation of calcium and phosphate ions at the interface. A structurally stable protein layer with incorporated calcium and phosphate ions at the implant-tissue interface could be an important prerequisite for enhanced bone formation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Electron Spectroscopy and Related Phenomena - Volume 216, April 2017, Pages 33-38
نویسندگان
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