کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5400021 1505913 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fluorescence quenching effect of guanine interacting with water-soluble cationic porphyrin
ترجمه فارسی عنوان
اثر گسیل فلورسانس گوانین در تعامل با پورفیرین کاتیون محلول در آب
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی تئوریک و عملی
چکیده انگلیسی
The process of association between 5,10,15,20-tetrakis[4-(trimethylammonio)phenyl]-21H,23H-porphine tetra-p-tosylate (H2TTMePP) and guanine has been studied both in NaOH solution and TRIS buffer analysing its absorption and steady-state fluorescence spectra. The fluorescence quenching effect observed during interactions porphyrin-guanine points at the fractional accessibility of the fluorophore for the quencher. The association and fluorescence quenching constants are of the order of magnitude of 105 mol−1. The fluorescence lifetimes and the quantum yields of the porphyrin monoanion form were established. The results demonstrate that guanine can interact with H2TTMePP at basic pH and through formation of stacking complexes is able to quench its ability to emission.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Luminescence - Volume 147, March 2014, Pages 27-33
نویسندگان
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