Determination of vitamin C in drugs using of an optimized novel TCPO-Amplex red-gold/silver alloy nanoparticles-H2O2 chemiluminescence method by the Box-Behnken design

► The Box-Behnken design was used to optimize peroxyoxalate-chemiluminescence system. ► Amplex red is as new fluorescent emitters for peroxyoxalate chemiluminescence. ► It is introduce a method for determination of vitamin C. ► Detection limit of vitamin C was obtained about 0.012 μg/mL.