کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5434986 1509148 2017 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In vitro biocompatibility of a ferrimagnetic glass-ceramic for hyperthermia application
ترجمه فارسی عنوان
بیومس انعطاف پذیری شیشه ای سرامیک فریم مگنتیک برای کاربرد هیپرترمی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
چکیده انگلیسی


- In vitro characterization of a ferrimagnetic glass-ceramic has been performed, before and after treatment in SBF.
- The SBF pre-treatment stimulates the cellular function and acts as a surface activation process, increasing cells activity.
- Pre-treatment with SBF annealed the reactivity towards C-H bond of formate.

Ferrimagnetic glass-ceramics containing magnetite crystals were developed for hyperthermia applications of solid neoplastic tissue. The present work is focused on in vitro evaluation of the biocompatibility of these materials, before and after soaking in a simulated body fluid (SBF). X-ray diffraction, scanning electron microscopy, atomic absorption spectrophotometry, X-ray photoelectron spectrometry and pH measurements were employed in glass-ceramic characterisation. The free-radical mediated reactivity of the glass-ceramic was evaluated by Electron Paramagnetic Resonance (EPR) spin trapping. Cell adhesion and proliferation tests were carried out by using 3T3 murine fibroblasts. Cytotoxicity was performed by qualitative evaluation of human bone osteosarcoma cells U2OS cell line. The results show that almost two times more 3T3 cells proliferated on the samples pre-treated in SBF, compared with the untreated specimens. Moreover a decrease of confluence was observed at 48 and 72 h for U2OS cells exposed to the untreated glass-ceramic, while the powder suspensions of glass-ceramic pre-treated in SBF did not influence the cell morphology up to 72 h of exposition. The untreated glass-ceramic exhibited Fenton-like reactivity, as well as reactivity towards formate molecule. After pre-treatment with SBF the reactivity towards formate was completely suppressed. The concentration of iron released into the SBF solution was below 0.1 ppm at 37 °C, during one month of soaking. The different in vitro behaviour of the samples before and after SBF treatment has been correlated to the bioactive glass-ceramic surface modifications as detected by morphological, structural and compositional analyses.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 73, 1 April 2017, Pages 778-787
نویسندگان
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