کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5445068 | 1511116 | 2017 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Biodegradation and Detoxification of Malachite Green Dye Using Novel Enzymes from Bacillus cereus Strain KM201428: Kinetic and Metabolite Analysis
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی انرژی
انرژی (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Enzyme based degradation of organic pollutants is a promising detoxifying approach due to the promiscuous nature of the enzyme, efficiency, cost effective and ecofriendly. In the present study, we have carried out detailed decoloration and degradation studies on a model triphenyl methane group of dyes (Malachite Green dye (MG)) using a newly isolated enzyme from Bacillus cereus KM201428 under the static condition. Biodegradation of dyes was monitored by UV-VIS spectrophotometer and the resultant metabolites analyzed by Liquid Chromatography-Hybrid Quadrupole Time of Flight Mass Spectrometry (LC-QToF-MS) and Gas Chromatography/Mass Spectrometry (GC - MS). Metabolite analysis results revealed that enzymatic degradation of MG dye resulted in complete mineralization and benzene ring-removal; the latter known for organic dye toxicity. Kinetic study results revealed that first-order kinetic model was best applicable for describing MG dye decoloration. Michaelise-Menten kinetics, Lineweaver-Burk plot and Eadie-Hofstee plot models were used to establish the kinetic parameters for the dye decoloration. Lineweaver-Burk plot provided the best theoretical correlation of the experimental data with maximum rate (Vmax) of 17.70 mg l-1h-1 and Michaelis constant (Km) of 124 mgl-1. Results provide evidence that crude enzyme from Bacillus cereus strain KM201428 offers an effective, renewable, ecofriendly and affordable biotechnology for treatment of industrial effluents polluted with organic dye.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Energy Procedia - Volume 119, July 2017, Pages 38-51
Journal: Energy Procedia - Volume 119, July 2017, Pages 38-51
نویسندگان
Wycliffe Chisutia Wanyonyi, John Mmari Onyari, Paul Mwanza Shiundu, Francis Jackim Mulaa,