کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5468960 | 1519053 | 2017 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Immobilization of Candida antarctica lipase B on kaolin and its application in synthesis of lipophilic antioxidants
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
علوم زمین و سیارات
ژئوشیمی و پترولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Lipase B from Candida antarctica was immobilized by adsorption onto laboratory prepared metakaolin. This support has great potential for enzyme immobilization since it has good mechanical characteristics and thermal stability and it is easily obtained from naturally abundant raw kaolin, as presented in this paper. Immobilization process was studied as a function of pH, ionic strength and protein concentration in terms of immobilization and activity yield. It was shown that immobilization occurred via the Langmuir model, and that electrostatic forces were major, but not exclusive contributor for the binding process. The optimum conditions were achieved in 10Â mM acetic buffer pHÂ 5. Biocatalyst obtained under optimum conditions was further successfully used in the synthesis of lipophilic antioxidants, where conversion yields as high as 100% were achieved.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Applied Clay Science - Volume 135, January 2017, Pages 103-111
Journal: Applied Clay Science - Volume 135, January 2017, Pages 103-111
نویسندگان
Sonja JakovetiÄ TanaskoviÄ, Bojan JokiÄ, Sanja GrbavÄiÄ, Ivana Drvenica, Nevena PrlainoviÄ, Nevena LukoviÄ, Zorica KneževiÄ-JugoviÄ,