کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5499007 | 1533483 | 2017 | 9 صفحه PDF | دانلود رایگان |
- UV radiation/illumination of chlorophyll leads to its irreversible degradation.
- Photodegradation is governed by photon energy input.
- Chlorophyll aggregation plays a protective role against UV radiation/illumination.
- Chlorophyll photodegradation is accompanied by products formation.
- Main chlorophyll products are pheophytin and hydroxy-pheophytin in early stages.
Irreversible chlorophyll degradation induced by continuous white light illumination and UV-B irradiation in the aqueous mediums (with 10%, 30% and 50% of methanol) was investigated using the ultrahigh liquid chromatography coupled with diode array and electrospray ionization mass spectrometry detectors (UHPLC-DAD-ESIMS). The degradation was governed by energy input of photons: higher energy of UV-B irradiation induced faster chlorophyll degradation and accordingly faster products formation in comparison to the white light treatment. Main light- or/and UV-B-induced products of chlorophyll in the aqueous mediums were hydroxy-pheophytin a, pheophytin a and hydroxy-lactone-pheophytin a, accompanied with the corresponding epimers. Chlorophylls aggregation dominant in the aqueous medium with the highest methanol content (50%) play a protective role against the UV-B radiation and white light illumination.
Journal: Radiation Physics and Chemistry - Volume 141, December 2017, Pages 8-16