کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5505233 | 1400263 | 2017 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Sensitivity of mitochondrial DNA depleted Ï0 cells to H2O2 depends on the plasma membrane status
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کلمات کلیدی
GPXRPMIMnSODPRXBSA - BSAAdenosine Triphosphate - آدنوزین تری فسفاتATP - آدنوزین تری فسفات یا ATPbovine serum albumin - آلبومین سرم گاوmanganese superoxide dismutase - سوپر اکسید دیسموتاز منگنزCatalase activity - فعالیت کاتالازRoswell Park Memorial Institute - مؤسسه یادبود پارک RoswellH2O2 - هیدروژن پراکسیدPlasma membrane potential - پتانسیل غشاء پلاسماPeroxiredoxin - پروکسی ردوکسینglutathione peroxidase - گلوتاتیون پراکسیداز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
To clarify the relationship between mitochondrial DNA (mtDNA)-depleted Ï0 cells and the cellular sensitivity to hydrogen peroxide (H2O2), we established HeLa and SAS Ï0 cell lines and investigated their survival rate in H2O2, radical scavenging enzymes, plasma membrane potential status, and chronological change in intracellular H2O2 amount under the existence of extracellular hydrogen peroxide compared with the parental cells. The results revealed that Ï0 cells had higher sensitivity to H2O2 than their parental cells, even though the catalase activity of Ï0 cells was up-regulated, and the membrane potential of the Ï0 cells was lower than their parental cells. Furthermore, the internal H2O2 amount significantly increased only in Ï0 cells after 50 μM H2O2 treatment for 1 h. These results suggest that plasma membrane status of Ï0 cells may cause degradation, and the change could lead to enhanced membrane permeability to H2O2. As a consequence, Ï0 cells have a higher H2O2 sensitivity than the parental cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 490, Issue 2, 19 August 2017, Pages 330-335
Journal: Biochemical and Biophysical Research Communications - Volume 490, Issue 2, 19 August 2017, Pages 330-335
نویسندگان
Kazuo Tomita, Yoshikazu Kuwahara, Yuko Takashi, Takao Tsukahara, Akihiro Kurimasa, Manabu Fukumoto, Yoshihiro Nishitani, Tomoaki Sato,