کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5505258 | 1400263 | 2017 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effect of calcium carbonate particle shape on phagocytosis and pro-inflammatory response in differentiated THP-1 macrophages
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کلمات کلیدی
Needle-shapedDMEMFBSphorbol 12-myristate 13-acetateIL-8Brunauer–Emmett–Teller - Brunauer-Emmett-TellerDulbecco's modified Eagle Medium - Eagle Medium اصلاح شده DulbeccoPMA - LDC هاAragonite - آراگونیتTem - این استInterleukin-8 - اینترلوکین -8tumor necrosis factor-alpha - تومور نکروز عامل آلفاPotassium titanate - تیتانات پتاسیمARA - در حال حاضرfetal bovine serum - سرم جنین گاوBET - شرطTNF-α - فاکتور نکروز توموری آلفاPhagocytosis - فاگوسیتوز یا بیگانهخواری Macrophage - ماکروفاژ SEM - مدل معادلات ساختاری / میکروسکوپ الکترونی روبشیScanning electron microscopy - میکروسکوپ الکترونی روبشیTransmission electron microscopy - میکروسکوپ الکترونی عبوریPro-inflammatory response - پاسخ مثبت التهابیSurface coating - پوشش سطحcal - کالCalcium carbonate - کربنات کلسیمCalcite - کلسیت
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Phagocytosis is a physiological process used by immune cells such as macrophages to actively ingest and destroy foreign pathogens and particles. It is the cellular process that leads to the failure of drug delivery carriers because the drug carriers are cleared by immune cells before reaching their target. Therefore, clarifying the mechanism of particle phagocytosis would have a significant implication for both fundamental understanding and biomedical engineering. As far as we know, the effect of particle shape on biological response has not been fully investigated. In the present study, we investigated the particle shape-dependent cellular uptake and biological response of differentiated THP-1 macrophages by using calcium carbonate (CaCO3)-based particles as a model. Transmission electron microscopy analysis revealed that the high uptake of needle-shaped CaCO3 particles by THP-1 macrophages because of their high phagocytic activity. In addition, the THP-1 macrophages exposed to needle-shaped CaCO3 accumulated a large amount of calcium in the intracellular matrix. The enhanced release of interleukin-8 (IL-8) and tumor necrosis factor-alpha (TNF-α) by the THP-1 macrophages suggested that the needle-shaped CaCO3 particles trigger a pro-inflammatory response. In contrast, no pro-inflammatory response was induced in undifferentiated THP-1 monocytes exposed to either needle- or cuboidal-shaped CaCO3 particles, probably because of their low phagocytic activity. We also found that phosphate-coated particles efficiently repressed cellular uptake and the resulting pro-inflammatory response in both THP-1 macrophages and primary peritoneal macrophages. Our results indicate that the pro-inflammatory response of macrophages upon exposure to CaCO3 particles is shape- and surface property-dependent, and is mediated by the intracellular accumulation of calcium ions released from phagocytosed CaCO3 particles.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 490, Issue 2, 19 August 2017, Pages 499-505
Journal: Biochemical and Biophysical Research Communications - Volume 490, Issue 2, 19 August 2017, Pages 499-505
نویسندگان
Yosuke Tabei, Sakiko Sugino, Kenichiro Eguchi, Masahiko Tajika, Hiroko Abe, Yoshihiro Nakajima, Masanori Horie,