کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5505552 | 1400273 | 2017 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
On the conformational stability of the smallest RNA kissing complexes maintained through two G·C base pairs
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Two identical 5â²GACG3â² tetra-loop motifs with different stem sequences (called H2 and H3) are found in the 5â² end region of Moloney Murine Leukemia Virus (MMLV) genomic RNA. They play important roles in RNA dimerization and encapsidation through two identical tetra-loops (5â²GACG3â²) forming a loop-to-loop kissing complex, the smallest RNA kissing complex ever found in nature. We examined the effects of a loop-closing base pair as well as a stem sequence on the conformational stability of the kissing complex. UV melting analysis and gel electrophoresis were performed on eight RNA sequences mimicking the H2 and H3 hairpin tetra-loops with variation in loop-closing base pairs. Our results show that changing the loop-closing base pair from the wildtype (5â²A·U3â² for H3, 5â²U·A3â² for H2) to 5â²G·C3'/5â²C·G3â² has significant effect on the stability of the kissing complexes: the substitution to 5â²C·G3â² significantly decreases both thermal and mechanical stability, while switching to the 5â²G·C3â² significantly increases the mechanical stability only. The kissing complexes with the wildtype loop-closing base pairs (5â²A·U3â² for H3 and 5â²U·A3â² for H2) show different stability when attached to a different stem sequence (H2 stem vs. H3 stem). This suggests that not only the loop-closing base pair itself, but also the stem sequence, affects the conformational stability of the RNA kissing complex.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 483, Issue 1, 29 January 2017, Pages 39-44
Journal: Biochemical and Biophysical Research Communications - Volume 483, Issue 1, 29 January 2017, Pages 39-44
نویسندگان
Wally Chu, Akila Weerasekera, Chul-Hyun Kim,