کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5506032 1400284 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Crystal structure of RNA polymerase II from Komagataella pastoris
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Crystal structure of RNA polymerase II from Komagataella pastoris
چکیده انگلیسی


- K. pastoris Pol II was crystallized in three different crystal forms.
- One of the structures showed a highly-closed conformation of the stalk and the clamp.
- Structural comparisons revealed the ranges of the stalk and clamp movements of Pol II.

RNA polymerase II (Pol II) is a 12-subunit protein complex that conducts the transcription of mRNA and some small RNAs. In this work, the crystal structure of Pol II from the methylotropic yeast Komagataella pastoris (Pichia pastoris) was determined. While the structure is highly homologous to that of Pol II from the budding yeast Saccharomyces cerevisiae, the stalk and clamp modules of the K. pastoris Pol II displayed large inward rotations, closing the central cleft to a greater extent than in the known S. cerevisiae Pol II structures. The conformational differences reflect the inherent flexibilities of the stalk and the clamp, as additional low-resolution structures of K. pastoris Pol II in different crystal forms revealed diverse stalk and clamp orientations. Comparisons with other eukaryotic/archaeal RNA polymerase structures in the Protein Data Bank revealed the distributions of the stalk and clamp orientations. The conformational plasticity should be essential for transcriptional functions and binding various regulatory factors.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 487, Issue 2, 27 May 2017, Pages 230-235
نویسندگان
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