کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5507074 1536899 2017 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody
چکیده انگلیسی


- A method of de novo protein sequencing of mouse mAb was developed.
- Humanization of a mouse monoclonal antibody has been performed.
- Antihuman CD34 antibody impairs the tube formation of HUVECs.
- CD34 could be a potential drug target for antiangiogenic therapy.

QBEND/10 is a mouse immunoglobulin lambda-chain monoclonal antibody with strict specificity against human hematopoietic progenitor cell antigen CD34. Our in vitro study showed that QBEND/10 impairs the tube formation of human umbilical vein endothelial cells (HUVECs), suggesting that the antibody may be of potential benefit in blocking tumor angiogenesis. We provided a de novo protein sequencing method through tandem mass spectrometry to identify the amino acid sequences in the variable heavy and light chains of QBEND/10. To reduce immunogenicity for clinical applications, QBEND/10 was further humanized using the resurfacing approach. We demonstrate that the de novo sequenced and humanized QBEND/10 retains the biological functions of the parental mouse counterpart, including the binding kinetics to CD34 and blockage of the tube formation of the HUVECs.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemistry and Biophysics Reports - Volume 9, March 2017, Pages 51-60
نویسندگان
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