کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5512467 1540223 2017 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization, antioxidant activity and immunomodulatory activity of polysaccharides from the swollen culms of Zizania latifolia
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Characterization, antioxidant activity and immunomodulatory activity of polysaccharides from the swollen culms of Zizania latifolia
چکیده انگلیسی


- Water and alkali extractable polysaccharide was prepared from swollen culms of Zizania latifolia.
- Their primary chemical compositions and triple helix conformation were determined.
- Their antioxidant and immunomudulatory activities were evaluated by in vitro assays.

The swollen culms of Zizania latifolia have been used as a vegetable and traditional herbal medicine in China, Japan, Korea and Southeast Asia countries. Up to date, there is little information about the polysaccharides from the swollen culms of Zizania latifolia and their potential bioactivities. In the present study, water extractable polysaccharide (ZLPs-W) and alkali extractable polysaccharide (ZLPs-A) was sequentially prepared from the swollen culms of Zizania latifolia. Both of ZLPs-W and ZLPs-A was found to be non-starch polydisperse heterpolysaccharide with β-type glycosidic linkage. ZLPs-W with triple helix conformation mainly composed of GalA, Glc and Gal. ZLPs-A without triple helix conformation mainly composed of Glc, Gal, Xyl and Ara. In in vitro antioxidant assay, ZLPs-W and ZLPs-A exhibited good scavenging activities. The EC50 of DPPH radical, superoxide radical and hydroxy radical scavenging activities for ZLPs-A is 1.87, 1.13 and 0.38 mg/mL compared with that for ZLPs-W is 2.95, 3.99 and 0.5 mg/mL, respectively. Moreover, in vitro cell assay revealed that ZLPs-W without cytotoxicity has higher immunomodulatory activity than ZLPs-A in terms of stimulation of phagocytic ability and NO production in murine macrophage RAW 264.7. At the treated concentration of 400 μg/mL and 100 μg/mL, ZLPs-W induced a highest phagocytosis index (1.76) and NO product (29.12 μmol/L), respectively. The results suggest that polysaccharide from the swollen culms of Zizania latifolia could be explored as potential natural antioxidant and immunomodulatory agents in medicine or functional food fields.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 95, February 2017, Pages 809-817
نویسندگان
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