کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5513043 | 1540975 | 2017 | 9 صفحه PDF | دانلود رایگان |
- Butyric acid alters the secretion of progesterone and estradiol in granulosa cells.
- Butyric acid effects the mRNA expression of GPR41/43 in porcine granulosa cells.
- Butyric acid adjusts the steroidogenic genes expression via cAMP signaling pathway.
Butyric acid (BA), one of the short chain fatty acids (SCFAs), has positive actions on the metabolism, inflammation, etc. However, whether it influences the reproductive physiology and if so the detail mechanism involved has not yet been determined. In this study, the porcine granulosa cells (PGCs) were treated with gradient concentrations of BA. After 24Â h culture, 0.05Â mM BA significantly stimulated the progesterone (P4) secretion (PÂ <Â 0.05), 5Â mM and 10Â mM BA significantly inhibited the P4 secretion (PÂ <Â 0.05). Simultaneously, BA up-regulated the estradiol (E2) secretion in a dose dependent manner, 5Â mM and 10Â mM BA significantly promoted the E2 level (PÂ <Â 0.05). In addition, 10Â mM BA significantly promoted the G-protein-coupled receptor 41/43 mRNA (PÂ <Â 0.05). Interestingly, 5Â mM BA treatment significantly down-regulated cyclic adenosine monophosphate (cAMP) content (PÂ <Â 0.05), steroidogenic acute regulatory (StAR), steroidogenic factor 1 (SF1), P450scc in the mRNA and/or protein level (PÂ <Â 0.05), and these actions were reversed by cAMP activator forskolin (FK). Moreover, the co-treatment of 5Â mM BA and bupivacaine (BPC, the cAMP inhibitor) significantly accumulated the inhibition action of BPC on cAMP, the secretion of P4, and the abundance of StAR mRNA (PÂ <Â 0.05), inhibited the up-regulation of 5Â mM BA on the E2 secretion (PÂ <Â 0.05). Further, the Global Proteome and KEGG pathway analysis found that 5Â mM BA significantly up-regulated the I3LM80 proteins (PÂ <Â 0.05), which is involved in the steroid biosynthesis signaling pathway. 5Â mM BA significantly decreased the F2Z5G3 protein level (PÂ <Â 0.05), and the cAMP signaling pathway. In conclusion, present findings for the first time demonstrated that BA could regulate the P4 and E2 hormone synthesis in PGCs via the cAMP signaling pathway.
Journal: The Journal of Steroid Biochemistry and Molecular Biology - Volume 172, September 2017, Pages 89-97