کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5514232 1541593 2017 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Nitrosoamphetamine binding to myoglobin and hemoglobin: Crystal structure of the H64A myoglobin-nitrosoamphetamine adduct
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Nitrosoamphetamine binding to myoglobin and hemoglobin: Crystal structure of the H64A myoglobin-nitrosoamphetamine adduct
چکیده انگلیسی


• Nitrosoamphetamine binds to the heme sites of hemoglobin (Hb) and myoglobin (Mb).
• The X-ray crystal structure of the H64A Mb-nitrosoamphetamine derivative has been determined.
• The Hb- and Mb-nitrosoamphetamine adducts are stable in air for extended periods.

N-hydroxyamphetamine (AmphNHOH) is an oxidative metabolite of amphetamine and methamphetamine. It is known to form inhibitory complexes upon binding to heme proteins. However, its interactions with myoglobin (Mb) and hemoglobin (Hb) have not been reported. We demonstrate that the reactions of AmphNHOH with ferric Mb and Hb generate the respective heme-nitrosoamphetamine derivatives characterized by UV-vis spectroscopy. We have determined the X-ray crystal structure of the H64A Mb-nitrosoamphetamine complex to 1.73 Å resolution. The structure reveals the N-binding of the nitroso-d-amphetamine isomer, with no significant H-bonding interactions between the ligand and the distal pocket amino acid residues.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Nitric Oxide - Volume 67, 1 July 2017, Pages 26–29