کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5515968 1542300 2018 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
CTA1: Purified and display onto gram-positive enhancer matrix (GEM) particles as mucosal adjuvant
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
CTA1: Purified and display onto gram-positive enhancer matrix (GEM) particles as mucosal adjuvant
چکیده انگلیسی


- CTA1 proteins fused to a PA anchor were purified and displayed on GEM particles.
- CTA1-GEM complexes exhibit enhanced mucosal as well as systemic immune responses.
- CTA1-GEM complexes were prepared using a time and cost efficient, one-step process.

The A1 subunit of cholera toxin (CTA1) retains the adjuvant function of CT, without its toxic side effects, making the molecule a promising mucosal adjuvant. However, the methods required to obtain a pure product are both complicated and expensive, constricting its potential commercial applicability. Here, we fused the peptidoglycan binding domain (PA) to the C-terminus of CTA1, which enabled the fusion protein to be expressed by Bacillus subtilis, and secreted into the culture medium. CTA1 was then purified and displayed on GEM particles using a one step process, which resulted in the formation of CTA1-GEM complexes. Next, the CTA1-GEM complexes were used as an adjuvant to enhance the immune responses of mice to the influenza subunit vaccine. It was observed that the CTA1-GEM complexes enhanced specific systemic (IgG) and mucosal (IgA) immune responses against antigen, and induced cellular immune responses as well. The data presented here suggests that CTA1-GEM complexes can serve as a viable mucosal adjuvant.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 141, January 2018, Pages 19-24
نویسندگان
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