کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
5515971 1400791 2018 5 صفحه PDF سفارش دهید دانلود کنید
عنوان انگلیسی مقاله
Heterologous expression of Trametes versicolor laccase in Saccharomyces cerevisiae
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Heterologous expression of Trametes versicolor laccase in Saccharomyces cerevisiae
چکیده انگلیسی


- The expression of Trametes versicolor laccase in Saccharomyces cerevisiae was optimized.
- The recombinant laccase was found to be a heavily hypermannosylated glycoprotein.
- The optimum pH and and thermo-stability of the recombinant laccase were not changed.

Laccase is used in various industrial fields, and it has been the subject of numerous studies. Trametes versicolor laccase has one of the highest redox potentials among the various forms of this enzyme. In this study, we optimized the expression of laccase in Saccharomyces cerevisiae. Optimizing the culture conditions resulted in an improvement in the expression level, and approximately 45 U/L of laccase was functionally secreted in the culture. The recombinant laccase was found to be a heavily hypermannosylated glycoprotein, and the molecular weight of the carbohydrate chain was approximately 60 kDa. These hypermannosylated glycans lowered the substrate affinity, but the optimum pH and thermo-stability were not changed by these hypermannosylated glycans. This functional expression system described here will aid in molecular evolutionary studies conducted to generate new variants of laccase.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 141, January 2018, Pages 39-43
نویسندگان
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