کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5517055 1543038 2017 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An inhibition enzyme immuno assay exploring recombinant invariant surface glycoprotein and monoclonal antibodies for surveillance of surra in animals
ترجمه فارسی عنوان
بررسی آنزیم مهارکننده ایمنی برای بررسی گلیکوپروتئین سطحی غیرارادی نوترکیب و آنتیبادیهای منوکلونال برای نظارت بر سوره در حیوانات
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
چکیده انگلیسی


- Monoclonal antibodies were engineered against recombinant ISG (rISG) of T. evansi.
- An inhibition immunoassay exploring MAbs and rISG was developed to diagnose surra.
- 98.8% diagnostic sensitivity and 99.2% specificity were found.
- A Panel of 1192 serum samples was tested with CATT and new assay for comparison.
- Chi-square and kappa value revealed agreement between developed test and CATT.

The present study is aimed at the development of inhibition ELISA (I-ELISA) exploring monoclonal antibodies (MAbs) and recombinant invariant surface glycoprotein. The extracellular domain (ED) of invariant surface glycoprotein (ISG-75) from Trypanosoma evasni has been heterologously expressed in Pichia pastoris (X-33). The recombinant ISG-75 (rISG-75ED) was characterized by immunoblot and ELISA, followed by the production of MAbs against rISG-75ED. The MAbs were characterized by immunoblot and then explored in the development of I-ELISA for the detection of surra. The diagnostic potential of the developed test has been evaluated using 1192 field sera sample including cattle, buffalo, donkey, horse and camel. The statistical analysis of the data showed optimum combination of diagnostic sensitivity and specificity at 98.8% and 99.2% respectively, with cut-off percentage inhibition (PI) value of >45. The Cohen's kappa coefficient of agreement was found to be 0.98. Hence, the diagnostic test developed in the present study can be exploited as a potential and reliable tool in the serodiagnosis and surveillance of surra in animals.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biologicals - Volume 46, March 2017, Pages 148-152
نویسندگان
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