کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
5518237 1401059 2017 3 صفحه PDF سفارش دهید دانلود کنید
عنوان انگلیسی مقاله
Genomics/technical resourcesIn-depth transcriptome analysis of Larimichthys polyactis, de novo assembly, functional annotation
موضوعات مرتبط
مهندسی و علوم پایه علوم زمین و سیارات علوم زمین و سیاره ای (عمومی)
پیش نمایش صفحه اول مقاله
Genomics/technical resourcesIn-depth transcriptome analysis of Larimichthys polyactis, de novo assembly, functional annotation
چکیده انگلیسی

Larimichthys polyactis (small yellow croaker) is a commercially important marine benthic fish, and is the main catching target of fisheries in China, Japan and Korea. Researches across different fields have progressed the understanding of this species; however, no genomics studies of this fish have been reported so far. In this study, we performed de novo transcriptome sequencing of eight cDNA libraries from brain, gill, heart, intestine, liver, muscle, ovary, and testis tissues of L. polyactis. A total of 182,227,948 paired-end reads (> 200 bp) were generated, from which 134,439 transcripts were assembled. These transcripts yielded a total of 93,990 non-redundant transcripts (unigenes) that were assigned functional annotations based on BlastX searches (E-value < 1 × 10−5) against the following databases: NCBI NR, Swiss-Prot, TrEMBL, CDD, Pfam, and COG. In addition, 12,539 simple sequence repeats (SSRs) were identified. Our study provides a valuable resource of L. polyactis transcriptomic data that are expected to be useful for gene expression and functional studies of L. polyactis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Marine Genomics - Volume 33, June 2017, Pages 27-29
نویسندگان
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