کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
5518453 1401069 2017 5 صفحه PDF سفارش دهید دانلود کنید
عنوان انگلیسی مقاله
A simple and fast method for extraction and quantification of cryptophyte phycoerythrin
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
A simple and fast method for extraction and quantification of cryptophyte phycoerythrin
چکیده انگلیسی

The microalgal pigment phycoerythrin (PE) is of commercial interest as natural colorant in food and cosmetics, as well as fluoroprobes for laboratory analysis. Several methods for extraction and quantification of PE are available but they comprise typically various extraction buffers, repetitive freeze-thaw cycles and liquid nitrogen, making extraction procedures more complicated. A simple method for extraction of PE from cryptophytes is described using standard laboratory materials and equipment. The cryptophyte cells on the filters were disrupted at −80 °C and added phosphate buffer for extraction at 4 °C followed by absorbance measurement. The cryptophyte Rhodomonas salina was used as a model organism.
- Simple method for extraction and quantification of phycoerythrin from cryptophytes.
- Minimal usage of equipment and chemicals, and low labor costs.
- Applicable for industrial and biological purposes.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: MethodsX - Volume 4, 2017, Pages 209-213
نویسندگان
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