کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5519203 | 1544098 | 2017 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development of an elisa for the diagnosis of reactive IgE antibodies anti-therapeutic horse sera
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کلمات کلیدی
intravenous IganaphylacticHuman immunoglobulin ECBSIVIgmAb(s) - mAb (s)Immunological assay - آزمایش ایمونولوژیکDiagnostic assay - آزمایش تشخیصیmonoclonal antibody(ies) - آنتی بادی منوکلونال (ها)enzyme linked immunosorbent assay - آنزیم تست ایمونوسیورسانس مرتبط استSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدSodium dodecyl sulfate polyacrylamide gel electrophoresis - الکتروفورز ژل پلی اتیل آمید سدیم دودسیل سولفاتELISA - تست الیزاcoefficient variation - تنوع ضریبHypersensitivity - حساسیت بیش از حدAntivenom - ضد زهر یا پادزهرmap - نقشهPolyacrylate - پلی آکریلMultiple antigen peptide - پپتید آنتیژن چندگانهhigh performance liquid chromatography - کروماتوگرافی مایع با کارایی بالاHPLC - کروماتوگرافی مایعی کارا
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Hypersensitive diseases that involve IgE reactivity are important concern of public, especially those encompassing the potential pathogenesis from the administration of horse serum-based therapeutics such as antivenoms. A method for the definitive diagnosis of reactive IgE is important for identifying allergic patients to control severe collateral effects during planned and emergency application of immunotherapies when the allergy source cannot be avoided for treatment. To date, no tests have been developed to accompany the wide range of antivenoms produced from horse sera. The aim of this was to develop a cost-effective ELISA of high sensitivity and specificity to detect circulating patient IgE that binds horse IgG3, the most prevalent antibody class in passive antibody therapies. Horse IgG3 was purified in a single step on jacalin-Sepharose and absorbed to standard ELISA plates as the capture molecule for reactive human IgE. The direct performance evaluation with allergenic and non-allergenic patient, together with competitive peptides assays, showed high sensitivity and specificity to detect human IgE that recognized horse IgG3. The analytical sensitivity and ED50 were calculated to be 0.01 μg mLâ1 and 0.052 μg mLâ1, respectively. The intra- and inter-assay coefficient of variation ranged from 3.3 to 11.1% and 4.0-8.0%, respectively. The horse IgG3-based ELISA assay can detect reactive allergenic IgE at picomolar concentrations. The coefficient of variation suggests that it can be easily standardized between laboratories, provide rapid and can be applied to population surveillance. Patient management during treatment for envenomation would be greatly improved by a robust and reliable diagnostic test for preexisting allergies to mitigate life-threating consequences of hypersensitivity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicon - Volume 138, November 2017, Pages 37-42
Journal: Toxicon - Volume 138, November 2017, Pages 37-42
نویسندگان
Salvatore Giovanni De-Simone, Andre Luis Almeida Souza, Aniesse Silva Aguiar, Anibal Raphel Melgarejo, David William Provance-Jr,