کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5521954 1545660 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research paperLong-term sustainable dendritic cell-specific depletion murine model for periodontitis research
ترجمه فارسی عنوان
مدل پریودنتال دندریتیک پایدار درازمدت برای بررسی پریودنتیت
کلمات کلیدی
موش، سلولهای دندریتیک، مدل های حیوانی، ناک اوت، توکسین دیفتری،
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
چکیده انگلیسی


- Diphtheria toxin (DT) can be used in conditional knockout mouse models.
- DT chronic exposure in zDC-DTR mice does not induce neutropenia/weight loss.
- Bone marrow Pre-DCs can be depleted for up to 4 weeks in zDC-DTR mice.
- Splenic conventional DCs were not significantly affected by DT treatment.

Dendritic cells (DCs) are specialized antigen-presenting cells that play a pivotal role in the pathogenesis of periodontitis. The use of animal models to study the role of DCs in periodontitis has been limited by lack of a method for sustained depletion of DCs. Hence, the objectives of this study were to validate the zDC-DTR knockin mouse model of conventional DCs (cDCs) depletion, as well as to investigate whether this depletion could be sustained long enough to induce alveolar bone loss in this model. zDC-DTR mice were treated with different dose regimens of diphtheria toxin (DT) to determine survival rate. A loading DT dose of 20 ng/bw, followed and maintained with doses of 10 ng/bm every 3 days for up to 4 weeks demonstrated 80% survival. Animals were weighed weekly and peripheral blood was obtained to confirm normal neutrophil counts. Five animals per group were euthanized at baseline, 24 h, 1 and 4 weeks. Bone marrow (BM), spleen (SP) and gingival tissue (GT) were harvested, and cells were isolated, separated and stained for Pre-DCs precursors (CD45R− MHCII+ CD11c+ Flt3+ CD172a+) in BM, cDCs (CD11c+ MHCII+ CD209+) in spleen, and DCs in GT (CD45R+ MHCII+ CD11c+ DC-SIGN/CD209+). Pre-DCs in BM were significantly depleted at 24 h and depletion maintained for up to 4 weeks, as compared to blank (PBS) controls. Circulating cDCs in spleen demonstrated a non-significant trend to deplete in 1 week with high variability among mice. GT also showed a similar non-significant trend to deplete in 24 h. The zDC-DTR model seems to be viable for evaluating the role of DCs immune homeostasis disruption and alveolar bone loss pathogenesis in response to long-term oral infection.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 449, October 2017, Pages 7-14
نویسندگان
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