کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
5522187 1401300 2017 3 صفحه PDF ندارد دانلود کنید
عنوان انگلیسی مقاله
NoteQuantification of biofilm biomass by staining: Non-toxic safranin can replace the popular crystal violet
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
NoteQuantification of biofilm biomass by staining: Non-toxic safranin can replace the popular crystal violet
چکیده انگلیسی

•Common staining methods for biofilm quantification were evaluated side-by-side.•Gram's crystal violet performed better than aqueous 0.5% crystal violet solution.•Safranin staining produced similar results as crystal violet, but with higher reproducibility.•We recommend Safranin staining based on high reproducibility and low toxicity.

Crystal violet staining is commonly used for quantification of biofilm formation, although it is highly toxic. Here we test safranin as a non-toxic replacement. Safranin staining provided similar results as crystal violet, but with higher reproducibility. We therefore recommend safranin staining for biofilm biomass quantification.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 141, October 2017, Pages 87-89
نویسندگان
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