|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5522250||1545908||2017||3 صفحه PDF||سفارش دهید||دانلود کنید|
• Flash chromatography is a low-cost approach to large scale pyoverdin purification.
• A dual column approach allows for desalting and separation.
• Results from P. putida GB1 show that several closely related structures are produced.
Our knowledge of the biological and environmental reactivity of siderophores is limited by the difficulty and cost of obtaining reasonable quantities by purification or synthesis. In this note, we describe a modified procedure for the low-cost, mg-scale purification of pyoverdin-type siderophores using a dual-flash chromatography (reverse-phase absorption and size exclusion) approach.
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Journal: Journal of Microbiological Methods - Volume 135, April 2017, Pages 11–13