کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
5522250 1401304 2017 3 صفحه PDF سفارش دهید دانلود کنید
عنوان انگلیسی مقاله
A two-column flash chromatography approach to pyoverdin production from Pseudomonas putida GB1
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
A two-column flash chromatography approach to pyoverdin production from Pseudomonas putida GB1
چکیده انگلیسی


• Flash chromatography is a low-cost approach to large scale pyoverdin purification.
• A dual column approach allows for desalting and separation.
• Results from P. putida GB1 show that several closely related structures are produced.

Our knowledge of the biological and environmental reactivity of siderophores is limited by the difficulty and cost of obtaining reasonable quantities by purification or synthesis. In this note, we describe a modified procedure for the low-cost, mg-scale purification of pyoverdin-type siderophores using a dual-flash chromatography (reverse-phase absorption and size exclusion) approach.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 135, April 2017, Pages 11–13