|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5522263||1401304||2017||3 صفحه PDF||سفارش دهید||دانلود کنید|
- A screening method for oxo-AVM was developed based on its reaction with DNPH.
- Combined with HPLC assay, regioselective AVM oxidizing microorganisms were obtained.
- It is simple and rapid for the preliminary screening of AVM oxidizing biocatalysts.
A spectrophotometric screening method for avermectin oxidizing microbes by determination of 4â³-oxo-avermectin was established based on the reaction between 4â³-oxo-avermectin and 2,4-dinitrophenylhydrazine. Combined with a gradient HPLC assay, microorganisms capable of regioselectively oxidizing avermectin to 4â³-oxo-avermectin were successfully obtained by this method.
Journal: Journal of Microbiological Methods - Volume 135, April 2017, Pages 93-95