کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
5522263 1401304 2017 3 صفحه PDF ندارد دانلود کنید
عنوان انگلیسی مقاله
A spectrophotometric screening method for avermectin oxidizing microorganisms
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
A spectrophotometric screening method for avermectin oxidizing microorganisms
چکیده انگلیسی

•A screening method for oxo-AVM was developed based on its reaction with DNPH.•Combined with HPLC assay, regioselective AVM oxidizing microorganisms were obtained.•It is simple and rapid for the preliminary screening of AVM oxidizing biocatalysts.

A spectrophotometric screening method for avermectin oxidizing microbes by determination of 4″-oxo-avermectin was established based on the reaction between 4″-oxo-avermectin and 2,4-dinitrophenylhydrazine. Combined with a gradient HPLC assay, microorganisms capable of regioselectively oxidizing avermectin to 4″-oxo-avermectin were successfully obtained by this method.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 135, April 2017, Pages 93-95
نویسندگان
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