کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
5522295 1401305 2017 3 صفحه PDF ندارد دانلود کنید
عنوان انگلیسی مقاله
NoteA single tube system for the detection of Mycobacterium tuberculosis DNA using gold nanoparticles based FRET assay
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
NoteA single tube system for the detection of Mycobacterium tuberculosis DNA using gold nanoparticles based FRET assay
چکیده انگلیسی

•AuNP-FRET assay was developed for the detection of MTB DNA.•The developed assay has a sensitivity of 98.6% and specificity of 90%.•The assay is able to differentiate MTB from nontuberculous mycobacteria (NTM).•The assay does not require DNA amplification and utilizes a single tube.•Assay turnaround time is 15 min.

The global combat against MTB is limited by challenges in accurate affordable detection. In this study, a rapid, affordable, single tube system for detection of unamplified MTB16s rDNA was developed. Utilizing a AuNP based FRET system, this assay achieved a sensitivity and specificity of 98.6% and 90% respectively.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 139, August 2017, Pages 165-167
نویسندگان
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