کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
5522295 1401305 2017 3 صفحه PDF ندارد دانلود کنید
عنوان انگلیسی مقاله
NoteA single tube system for the detection of Mycobacterium tuberculosis DNA using gold nanoparticles based FRET assay
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی
پیش نمایش صفحه اول مقاله
NoteA single tube system for the detection of Mycobacterium tuberculosis DNA using gold nanoparticles based FRET assay
چکیده انگلیسی

•AuNP-FRET assay was developed for the detection of MTB DNA.•The developed assay has a sensitivity of 98.6% and specificity of 90%.•The assay is able to differentiate MTB from nontuberculous mycobacteria (NTM).•The assay does not require DNA amplification and utilizes a single tube.•Assay turnaround time is 15 min.

The global combat against MTB is limited by challenges in accurate affordable detection. In this study, a rapid, affordable, single tube system for detection of unamplified MTB16s rDNA was developed. Utilizing a AuNP based FRET system, this assay achieved a sensitivity and specificity of 98.6% and 90% respectively.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 139, August 2017, Pages 165-167
نویسندگان
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